Coelenterazine-v

Benz[f]imidazo[1,2-a]quinoxalin-3(11H)-one,8-hydroxy-2-[(4-hydroxyphenyl)methyl]-12-(phenylmethyl)-

Coelenterazine-v is a coelenterazine analog that emits a red fluorescent light in the presence of calcium (Ca2+). It is used as a reporter molecule for intracellular calcium concentration. Coelenterazine-v has been used in vitro to detect protein–protein interactions and in animal experiments to detect luciferase activity. Other coelenterazine-v analogs have also been developed with different color properties, including blue, green, yellow, orange, and purple. All these analogs, including coelenterazine-v, are used for optical imaging of cells or tissues or for detecting the activity of luciferases.